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(Plasmid #179081)


Item Catalog # Description Quantity Price (USD)
Plasmid 179081 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 11803
  • Total vector size (bp) 13136
  • Modifications to backbone
    addition of 3' and 5' piggyBac repeats, addition of pU6-pegRNA(B12B) sequence, addition of CAG promoter, addition of bGH (pA)
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology ; piggyBac transposon
  • Selectable markers
    Puromycin ; thymidine kinase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Growth instructions
    It is highly recommended to use NEB Stable at 30°C. Cloning efficiency and stability of plasmid can be compromised when using other strains / higher temperatures!
  • Copy number


  • Gene/Insert name
    SpCas9 SpG_H840A-PE2-MMLV-RT(dBB)-P2A-PAC_dTK(dBB)
  • Species
  • Insert Size (bp)
  • Mutation
    D1135L, S1136W, G1218K, E1219Q, R1335Q, T1337R
  • Promoter CAG
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    1378 bp of Cas9 SpG was PCR amplified from pCMV-PE2-SpG (Addgene Plasmid #159978)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-PE SpG was a gift from Tobias Cantz (Addgene plasmid # 179081 ; ; RRID:Addgene_179081)
  • For your References section:

    A selectable all-in-one CRISPR prime editing piggyBac transposon allows for highly efficient gene editing in human cell lines. Eggenschwiler R, Gschwendtberger T, Felski C, Jahn C, Langer F, Sterneckert J, Hermann A, Luhmann J, Steinemann D, Haase A, Martin U, Petri S, Cantz T. Sci Rep. 2021 Nov 12;11(1):22154. doi: 10.1038/s41598-021-01689-2. 10.1038/s41598-021-01689-2 PubMed 34773059