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p23-mNeongreen-DDX21 HGV
(Plasmid #179230)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 179230 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 179230-D50 50 μg of DNA in Tris buffer $495
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DNA 179230-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pHAGE-EF1a-IRES-ZsGreen
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    DDX21
  • Species
    H. sapiens (human)
  • Mutation
    D338H, E339G
  • Entrez Gene
    DDX21 (a.k.a. GUA, GURDB, II/Gu, RH, RH II/Gu, RH-II/GU, RH-II/GuA, gu-alpha)
  • Tag / Fusion Protein
    • mNeongreen (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p23-mNeongreen-DDX21 HGV was a gift from Ling-Ling Chen (Addgene plasmid # 179230 ; http://n2t.net/addgene:179230 ; RRID:Addgene_179230)

  • For your References section:

    lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription. Wu M, Xu G, Han C, Luan PF, Xing YH, Nan F, Yang LZ, Huang Y, Yang ZH, Shan L, Yang L, Liu J, Chen LL. Science. 2021 Jul 30;373(6554):547-555. doi: 10.1126/science.abf6582. 10.1126/science.abf6582 PubMed 34326237
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