pDEINO1
(Plasmid #179472)

• Purpose: Multi-host shuttle plasmid for D. radiodurans, E. coli, S. cerevisiae, S. meliloti, and P. tricornutum; contains an oriT for transfer via conjugation
• Depositing Lab: Bogumil Karas
• Publication: Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7.

Backbone

• Vector backbone: pAGE3.0
• Backbone manufacturer: Brumwell et al.
• Backbone size w/o insert (bp): 17515
• Total vector size (bp): 22148
• Modifications to backbone: Addition of D. radiodurans origin of replication and codon-optimized chloramphenicol resistance gene.
• Vector type: Synthetic Biology ; Bacterial and Yeast Cloning
• Selectable markers: HIS3 ; Chloramphenicol - D. radiodurans, E. coli; Neomycin/Kan - D. radiodurans, E. coli, S. meliloti; Nourseothricin (NAT) - algae

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Epi300
• Growth instructions: Plasmid can be induced to high copy with arabinose.
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: ori
• Alt name: D. radiodurans origin of replication
• Species: D. radiodurans
• Insert Size (bp): 3810

Gene/Insert 2

• Gene/Insert name: cat
• Alt name: Chloramphenicol resistance gene codon-optimized for D. radiodurans
• Species: Synthetic
• Insert Size (bp): 823

Resource Information

• Supplemental Documents:
  • pDEINO1-sequence.gb
• A portion of this plasmid was derived from a plasmid made by: Mary Lidstrom's Lab at the University of Washington (D. radiodurans origin of replication was cloned from pRAD1) and Murray Junop's Lab at Western University (D. radiodurans codon-optimized chloramphenicol gene was cloned from pRadDEST-GFP). Reference: Meima, R., & Lidstrom, M. E. (2000). Characterization of the minimal replicon of a cryptic Deinococcus radiodurans SARK plasmid and development of versatile Escherichia coli-D. radiodurans shuttle vectors. Applied and environmental microbiology, 66(9), 3856-3867.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry