pDEINO3
(Plasmid #179487)

• Purpose: Multi-host shuttle plasmid for E. coli, D. radiodurans, and S. cerevisiae; contains an oriT for transfer via conjugation
• Depositing Lab: Bogumil Karas
• Publication: Brumwell et al ACS Synth Biol. 2022 Mar 18;11(3):1068-1076. doi: 10.1021/acssynbio.1c00524. Epub 2022 Mar 7.

Backbone

• Vector backbone: pDEINO1
• Backbone manufacturer: Brumwell et al.
• Backbone size w/o insert (bp): 14930
• Total vector size (bp): 17215
• Modifications to backbone: Addition of tetracycline resistance gene. Removal of algae selective marker (Nourseothricin), S. meliloti origin of replication (RepA2B2C2) and NptII selective marker.
• Vector type: Synthetic Biology ; Bacterial and Yeast Cloning
• Selectable markers: HIS3 ; Chloramphenicol - D. radiodurans, E. coli; Tetracycline - D. radiodurans, E. coli

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Epi300
• Growth instructions: Plasmid can be induced to high copy with arabinose.
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: TetR/A
• Alt name: Tetracycline resistance gene
• Species: Pseudomonas aeruginosa
• Insert Size (bp): 2285

Resource Information

• Supplemental Documents:
  • pdeino3.gb
• A portion of this plasmid was derived from a plasmid made by: Jones and Gutterson (Tetracycline resistance gene was cloned from pRK7813). Reference: Jones, J. D., & Gutterson, N. (1987). An efficient mobilizable cosmid vector, pRK7813, and its use in a rapid method for marker exchange in Pseudomonas fluorescens strain HV37a. Gene, 61(3), 299-306.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry