ND4.2-Right DdCBE-G1397-C- T1413I
(Plasmid
#179685)
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PurposeExpression of base editor in mammalian cells
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179685 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCMV
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSOD2 MTS_3xHA_ND4.2 Right TALE_2aa_DddA G1397-C (T1413I)_1xUGI_SOD2 3'UTR
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SpeciesSynthetic
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MutationT1413I
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ND4.2-Right DdCBE-G1397-C- T1413I was a gift from David Liu (Addgene plasmid # 179685 ; http://n2t.net/addgene:179685 ; RRID:Addgene_179685) -
For your References section:
CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA. Mok BY, Kotrys AV, Raguram A, Huang TP, Mootha VK, Liu DR. Nat Biotechnol. 2022 Sep;40(9):1378-1387. doi: 10.1038/s41587-022-01256-8. Epub 2022 Apr 4. 10.1038/s41587-022-01256-8 PubMed 35379961
