CRISPR-free base editors with enhanced activity and expanded targeting scope in mitochondrial and nuclear DNA.
Mok BY, Kotrys AV, Raguram A, Huang TP, Mootha VK, Liu DR
Nat Biotechnol. 2022 Sep;40(9):1378-1387. doi: 10.1038/s41587-022-01256-8. Epub 2022 Apr 4. PubMed Article
Mok BY, Kotrys AV, Raguram A, Huang TP, Mootha VK, Liu DR
Nat Biotechnol. 2022 Sep;40(9):1378-1387. doi: 10.1038/s41587-022-01256-8. Epub 2022 Apr 4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 179678 | AP1 | Accessory plasmids |
| 179679 | CP2-TCC | Complementary plasmid |
| 179680 | ND4.2-Left TALE-G1397-N-DddA6 | Expression of base editor in mammalian cells |
| 179681 | ND4.2-Left TALE-G1397-N-DddA11 | Expression of base editor in mammalian cells |
| 179682 | ND4.2-Left TALE-G1397-N-DddA11-mCherry | Expression of base editor in mammalian cells and for cell sorting |
| 179683 | ND4.2-Left TALE-G1397-N-dead-mCherry | Expression of base editor in mammalian cells and for cell sorting |
| 179684 | ND4.2-Left TALE-G1397-N-wildtype | Expression of base editor in mammalian cells |
| 179685 | ND4.2-Right DdCBE-G1397-C- T1413I | Expression of base editor in mammalian cells |
| 179686 | ND4.2-Right DdCBE-G1397-C- T1413I-GFP | Expression of base editor in mammalian cells and for cell sorting |
| 179687 | ND4.2-Right DdCBE-G1397-C-wildtype | Expression of base editor in mammalian cells |
| 179688 | pBM10a | Target plasmid for context profiling |
| 179689 | pBM13g-sd4U | Bacterial expression of base editor |
| 179690 | pBM13h-sd4U | Bacterial expression of base editor |
| 179691 | pBM13-sd4U | Bacterial expression of base editor |
| 179692 | pBM19-sd4U | Bacterial expression of base editor |
| 179693 | pJC175e-DddI | Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein |