Purposevisualization of centromere heterochromatin
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||179701||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameheterochromatin protein alpha
SpeciesH. sapiens (human)
Entrez GeneCBX5 (a.k.a. HEL25, HP1, HP1A)
/ Fusion Protein
- EGFP (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
For more information, please refer to Sugimoto et al. (2001) Cell Struct Funct. https://doi.org/10.1247/csf.26.705
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pEGFP-HP1alpha was a gift from Kenji Sugimoto (Addgene plasmid # 179701 ; http://n2t.net/addgene:179701 ; RRID:Addgene_179701)
For your References section:Functional domain structure of human heterochromatin protein HP1(Hsalpha): involvement of internal DNA-binding and C-terminal self-association domains in the formation of discrete dots in interphase nuclei. Yamada T, Fukuda R, Himeno M, Sugimoto K. J Biochem. 1999 Apr . 125(4):832-7. 10.1093/oxfordjournals.jbchem.a022356 PubMed 10101299