pEGFP-HP1alpha
(Plasmid
#179701)
-
Purposevisualization of centromere heterochromatin
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 179701 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backbonepEGFP-C1
-
Vector typeMammalian Expression
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameheterochromatin protein alpha
-
SpeciesH. sapiens (human)
-
Entrez GeneCBX5 (a.k.a. HEL25, HP1, HP1A)
-
Tag
/ Fusion Protein
- EGFP (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
For more information, please refer to Sugimoto et al. (2001) Cell Struct Funct. https://doi.org/10.1247/csf.26.705
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEGFP-HP1alpha was a gift from Kenji Sugimoto (Addgene plasmid # 179701 ; http://n2t.net/addgene:179701 ; RRID:Addgene_179701) -
For your References section:
Functional domain structure of human heterochromatin protein HP1(Hsalpha): involvement of internal DNA-binding and C-terminal self-association domains in the formation of discrete dots in interphase nuclei. Yamada T, Fukuda R, Himeno M, Sugimoto K. J Biochem. 1999 Apr . 125(4):832-7. 10.1093/oxfordjournals.jbchem.a022356 PubMed 10101299
