pDsRed-HP1alpha
(Plasmid #179702)

• Purpose: visualization of centromere heterochromatin
• Depositing Lab: Kenji Sugimoto
• Publication: Nakagawa et al Biosci Biotechnol Biochem. 2014;78(4):556-64. doi: 10.1080/09168451.2014.893184. Epub 2014 May 9.

Backbone

• Vector backbone: pDsRed-C1
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: heterochromatin protein alpha
• Species: H. sapiens (human)
• Entrez Gene: CBX5 (a.k.a. HEL25, HP1, HP1A, HP1alpha)
• Tag / Fusion Protein:
  • dsRed (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry