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pmCherry-HP1alpha
(Plasmid #179703)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 179703 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    heterochromatin protein alpha
  • Species
    H. sapiens (human)
  • Entrez Gene
    CBX5 (a.k.a. HEL25, HP1, HP1A)
  • Tag / Fusion Protein
    • mCherry (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

NheI-BglII-digested PCR products pREST-B-mCherry into the corresponding sites of the vector backbone of pEGFP-C1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherry-HP1alpha was a gift from Kenji Sugimoto (Addgene plasmid # 179703 ; http://n2t.net/addgene:179703 ; RRID:Addgene_179703)
  • For your References section:

    Characterization of nucleolar localization and exclusion signals in terminal deoxynucleotidyltransferase interacting factor 2/estrogen receptor alpha-binding protein. Fukada T, Shibata S, Ueda T, Sasaki K, Shimoida Y, Senda-Murata K, Sugimoto K. Biosci Biotechnol Biochem. 2019 Jul;83(7):1255-1262. doi: 10.1080/09168451.2019.1591265. Epub 2019 Mar 24. 10.1080/09168451.2019.1591265 PubMed 30907250