TrpLE-M-nMSP1E3D1
(Plasmid
#179949)
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PurposeTagless membrane scaffold protein (MSP) for formation of nanodiscs.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 179949 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepET-24a(+)
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Backbone manufacturerGene Universal
- Backbone size w/o insert (bp) 5310
- Total vector size (bp) 6323
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsBL21(DE3) cells
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameTrpLE-M-nMSP1E3D1
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SpeciesSynthetic; gene
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Insert Size (bp)771
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MutationNative Trp residues have been mutated to Phe residues. Native Met residues have been mutated to Leu residues. A single methionine residue is placed between the TrpLE & MSP fusion to facilitate cleavage by cyanogen bromide.
- Promoter T7
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Tag
/ Fusion Protein
- TrpLE (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site NdeI (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer T7 term (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byGene Universal Inc. 200 Continental Dr. Suite 401, office 402, Newark DE 19713
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
TrpLE-M-nMSP1E3D1 was a gift from Kerney Glover (Addgene plasmid # 179949 ; http://n2t.net/addgene:179949 ; RRID:Addgene_179949) -
For your References section:
Facile production of tagless membrane scaffold protein for nanodiscs. Julien JA, Mutchek SG, Fernandez MG, Glover KJ. Anal Biochem. 2021 Nov 27:114497. doi: 10.1016/j.ab.2021.114497. 10.1016/j.ab.2021.114497 PubMed 34848201