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TrpLE-M-nMSP1E3D1
(Plasmid #179949)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 179949 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET-24a(+)
  • Backbone manufacturer
    Gene Universal
  • Backbone size w/o insert (bp) 5310
  • Total vector size (bp) 6323
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    BL21(DE3) cells
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TrpLE-M-nMSP1E3D1
  • Species
    Synthetic; gene
  • Insert Size (bp)
    771
  • Mutation
    Native Trp residues have been mutated to Phe residues. Native Met residues have been mutated to Leu residues. A single methionine residue is placed between the TrpLE & MSP fusion to facilitate cleavage by cyanogen bromide.
  • Promoter T7
  • Tag / Fusion Protein
    • TrpLE (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer T7
  • 3′ sequencing primer T7 term
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Gene Universal Inc. 200 Continental Dr. Suite 401, office 402, Newark DE 19713

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    TrpLE-M-nMSP1E3D1 was a gift from Kerney Glover (Addgene plasmid # 179949 ; http://n2t.net/addgene:179949 ; RRID:Addgene_179949)
  • For your References section:

    Facile production of tagless membrane scaffold protein for nanodiscs. Julien JA, Mutchek SG, Fernandez MG, Glover KJ. Anal Biochem. 2021 Nov 27:114497. doi: 10.1016/j.ab.2021.114497. 10.1016/j.ab.2021.114497 PubMed 34848201