AAV.CBA.YFP.miR-E_shAtxn1
(Plasmid
#180387)
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PurposeProducing AAV that encodes mouse Atxn1 shRNA with miR-E backbone
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 180387 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneAAV.CBA.YFP.miR-E
- Backbone size w/o insert (bp) 6337
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Vector typeAAV, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameshAtxn1
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gRNA/shRNA sequenceACGAACTGAAGTTTCCAGAAAA
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SpeciesM. musculus (mouse)
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Insert Size (bp)97
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Entrez GeneAtxn1 (a.k.a. 2900016G23Rik, Atx1, Gm10786, Sca1)
- Promoter CBA
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer ACTACCTGAGCTACCAGTCC
- 3′ sequencing primer CATAGCGTAAAAGGAGCAACA (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
AAV.CBA.YFP.miR-E_shAtxn1 was a gift from Huda Zoghbi (Addgene plasmid # 180387 ; http://n2t.net/addgene:180387 ; RRID:Addgene_180387) -
For your References section:
Dual targeting of brain region-specific kinases potentiates neurological rescue in Spinocerebellar ataxia type 1. Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR, Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi HY. EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. Epub 2021 Mar 11. 10.15252/embj.2020106106 PubMed 33709453
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