Dual targeting of brain region-specific kinases potentiates neurological rescue in Spinocerebellar ataxia type 1.
Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR, Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi HY
EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. Epub 2021 Mar 11. PubMed Article
Lee WS, Lavery L, Rousseaux MWC, Rutledge EB, Jang Y, Wan YW, Wu SR, Kim W, Al-Ramahi I, Rath S, Adamski CJ, Bondar VV, Tewari A, Soleimani S, Mota S, Yalamanchili HK, Orr HT, Liu Z, Botas J, Zoghbi HY
EMBO J. 2021 Apr 1;40(7):e106106. doi: 10.15252/embj.2020106106. Epub 2021 Mar 11. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 180379 | W118-1_eGFP | lentiviral transduction of eGFP gene |
| 180380 | W118-1_hZBTB7B | lentiviral transduction of human ZBTB7B gene |
| 180381 | W118-1_hZBTB7B_R363L | lentiviral transduction of human ZBTB7B gene with a point mutation (R363L) in the first ZF domain |
| 180382 | W118-1_hZBTB7B_R49H | lentiviral transduction of human ZBTB7B gene with a point mutation (R49H) in the BTB domain |
| 180383 | W118-1_hZBTB7B_D35N_R49Q | lentiviral transduction of human ZBTB7B gene with point mutations (D35N and R49Q) in the BTB domain |
| 180384 | W118-1_hRSK3(RPS6KA2) | lentiviral transduction of human RSK3 (RPS6KA2) gene |
| 180385 | W118-1_hRSK3(RPS6KA2)_K91A_K444A | lentiviral transduction of human RSK3 (RPS6KA2) gene with point mutations (K91A and K444A) that inactivate kinase activity |
| 180386 | AAV.CBA.YFP.miR-E_shControl | Producing AAV that encodes negative control shRNA with miR-E backbone |
| 180387 | AAV.CBA.YFP.miR-E_shAtxn1 | Producing AAV that encodes mouse Atxn1 shRNA with miR-E backbone |
| 180388 | AAV.CBA.YFP.miR-E_shRps6ka2-1 | Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-1 with miR-E backbone |
| 180389 | AAV.CBA.YFP.miR-E_shRps6ka2-2 | Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-2 with miR-E backbone |
| 180390 | AAV.CBA.YFP.miR-E_shRps6ka2-3 | Producing AAV that encodes mouse Rps6ka2 (Rsk3) shRNA-3 with miR-E backbone |