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pTCF79 H-2D(b) (CD8 mut)
(Plasmid #180453)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 180453 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET24a+
  • Backbone manufacturer
    Novagen
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    H-2D(b) (CD8 mut)
  • Species
    M. musculus (mouse)
  • Mutation
    D227K, replacement of transmembrane domain with enzymatic biotinylation site
  • GenBank ID
    NP_034510.3
  • Tag / Fusion Protein
    • BSP41 (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation prevents binding of CD8 (Potter et al. (1989) Nature doi.org/10.1038/337073a0). For detailed protocols of expression, purification and use to visualize antigen-specific T cells, please refer to Altman and Davis (2016) Curr. Protoc. Immunol. doi.org/10.1002/cpim.14. We recommend the E. coli BL21(DE3) strain for expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTCF79 H-2D(b) (CD8 mut) was a gift from John Altman (Addgene plasmid # 180453 ; http://n2t.net/addgene:180453 ; RRID:Addgene_180453)