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(Plasmid #180757)


Item Catalog # Description Quantity Price (USD)
Plasmid 180757 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    EMD Biosciences
  • Backbone size w/o insert (bp) 5199
  • Total vector size (bp) 5928
  • Modifications to backbone
    Removed lacO sequence, N-terminal His tag, and portion of the MCS to insert sfGFP with C-terminal S-tag
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy


  • Gene/Insert name
    superfolder green fluorescent protein
  • Alt name
  • Insert Size (bp)
  • Tag / Fusion Protein
    • strep tag (C terminal on insert)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    derived from pJL1-sfGFP, a gift from Michael Jewett (Addgene plasmid # 102634)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET30-T7-sfGFP was a gift from Michael Black (Addgene plasmid # 180757 ; ; RRID:Addgene_180757)
  • For your References section:

    Characterizing and Improving pET Vectors for Cell-free Expression. Jew K, Smith PEJ, So B, Kasman J, Oza JP, Black MW. Front Bioeng Biotechnol. 2022 Jun 23;10:895069. doi: 10.3389/fbioe.2022.895069. eCollection 2022. 10.3389/fbioe.2022.895069 PubMed 35814024