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(Plasmid #181854)


Item Catalog # Description Quantity Price (USD)
Plasmid 181854 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 5360
  • Total vector size (bp) 7304
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    DnaJB1(1-70; H33Q)-PKAcat(16-351)-mCherry
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • Mutation
    Histidine 33 in DnaJB1 changed to glutamine.
  • Entrez Gene
    PRKACA (a.k.a. CAFD1, PKACA, PPNAD4)
  • Entrez Gene
    DNAJB1 (a.k.a. HSPF1, Hdj1, Hsp40, RSPH16B, Sis1)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site HindIII (not destroyed)
  • 3′ cloning site XbaI (not destroyed)
  • 5′ sequencing primer CMV forward
  • 3′ sequencing primer BGH reverse
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1(+)-DnaJB1(H33Q)-PKAcat-mCherry was a gift from Jin Zhang (Addgene plasmid # 181854 ; ; RRID:Addgene_181854)
  • For your References section:

    Phase Separation of a PKA Regulatory Subunit Controls cAMP Compartmentation and Oncogenic Signaling. Zhang JZ, Lu TW, Stolerman LM, Tenner B, Yang JR, Zhang JF, Falcke M, Rangamani P, Taylor SS, Mehta S, Zhang J. Cell. 2020 Sep 17;182(6):1531-1544.e15. doi: 10.1016/j.cell.2020.07.043. Epub 2020 Aug 25. 10.1016/j.cell.2020.07.043 PubMed 32846158