pCAG-FLAG-IRES-blasticidin-Sox2 WT
(Plasmid
#182046)
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Purposeexpression of Sox2
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Depositing Lab
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Depositing OrganizationNational Cancer Center (KR)
Located in Republic of Korea -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 182046 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 182046-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 182046-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCAG-FLAG-IRES-blasticidin
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSox2
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SpeciesM. musculus (mouse)
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Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
- Promoter CAG
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Tag
/ Fusion Protein
- FLAG (N terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a
- (Common Sequencing Primers)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 182046-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 182046-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCAG-FLAG-IRES-blasticidin-Sox2 WT was a gift from Hyonchol Jang (Addgene plasmid # 182046 ; http://n2t.net/addgene:182046 ; RRID:Addgene_182046) -
For your References section:
O-GlcNAcylation of Sox2 at threonine 258 regulates the self-renewal and early cell fate of embryonic stem cells. Kim DK, Lee JS, Lee EY, Jang H, Han S, Kim HY, Hwang IY, Choi JW, Shin HM, You HJ, Youn HD, Jang H. Exp Mol Med. 2021 Nov;53(11):1759-1768. doi: 10.1038/s12276-021-00707-7. Epub 2021 Nov 24. 10.1038/s12276-021-00707-7 PubMed 34819616
