Purposeexpression of Sox2
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||182046||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Entrez GeneSox2 (a.k.a. Sox-2, lcc, ysb)
- Promoter CAG
/ Fusion Protein
- FLAG (N terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer n/a (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCAG-FLAG-IRES-blasticidin-Sox2 WT was a gift from Hyonchol Jang (Addgene plasmid # 182046 ; http://n2t.net/addgene:182046 ; RRID:Addgene_182046)
For your References section:O-GlcNAcylation of Sox2 at threonine 258 regulates the self-renewal and early cell fate of embryonic stem cells. Kim DK, Lee JS, Lee EY, Jang H, Han S, Kim HY, Hwang IY, Choi JW, Shin HM, You HJ, Youn HD, Jang H. Exp Mol Med. 2021 Nov;53(11):1759-1768. doi: 10.1038/s12276-021-00707-7. Epub 2021 Nov 24. 10.1038/s12276-021-00707-7 PubMed 34819616