PurposeTo convert human skin fibroblasts into induced basal forebrain cholinergic neurons (hiBFCNs) in combination with LHX8, GBX1, FGF2 and two small molecules, forskolin and LDN-193189
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||182234||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
Copy numberHigh Copy
SpeciesH. sapiens (human), M. musculus (mouse)
Insert Size (bp)2500
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
- 3′ cloning site unknown (unknown if destroyed)
- 5′ sequencing primer agctcgtttagtgaaccgtcagatc
- 3′ sequencing primer gagcaacatagttaagaataccag (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCSC-ASCL1-IRES-Sox11 was a gift from Chun-Li Zhang (Addgene plasmid # 182234 ; http://n2t.net/addgene:182234 ; RRID:Addgene_182234)
For your References section:Aging-relevant human basal forebrain cholinergic neurons as a cell model for Alzheimer's disease. Ma S, Zang T, Liu ML, Zhang CL. Mol Neurodegener. 2020 Oct 21;15(1):61. doi: 10.1186/s13024-020-00411-6. 10.1186/s13024-020-00411-6 PubMed 33087140