pDronpa-N1-Tau-μDpTT
(Plasmid #182323)

• Purpose: Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
• Depositing Lab: Jin Zhang
• Publication: Lin et al J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13.

Backbone

• Vector backbone: pDronpa-N1
• Backbone size w/o insert (bp): 3984
• Total vector size (bp): 6450
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Tau-μDronpa-TagRFP-T
• Alt name: Tau-Dronpa(H193T)-DEVD-TagRFP-T
• Insert Size (bp): 2466
• Mutation: Dronpa contains H193T mutation.
• Promoter: CMV
• Tag / Fusion Protein:
  • MAP tau (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XhoI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: CMV forward
• 3′ sequencing primer: SV40pA reverse

Resource Information

• Supplemental Documents:
  • pDronpa-N1-tau-Dp(H193T)-TT.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Evrogen Limited Use Label License for FPs
• Industry Terms:
  • Not Available to Industry