Addgene: DrFLINC Contextualizes Super-resolution Activity Imaging.

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Jin Zhang
Lin et al

DrFLINC Contextualizes Super-resolution Activity Imaging.
Lin W, Mo GCH, Mehta S, Zhang J
J Am Chem Soc. 2021 Sep 22;143(37):14951-14955. doi: 10.1021/jacs.1c05530. Epub 2021 Sep 13. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
182321	pcDNA3-Lyn-μDpTT	Plasma membrane-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182322	pcDNA3-Lifeact-μDpTT	Actin-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182323	pDronpa-N1-Tau-μDpTT	Microtubule-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182324	pcDNA3-DAKAP-μDpTT	Mitochondria-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182325	pcDNA3-CYP450-μDpTT	Endoplasmic reticulum-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182326	pcDNA3-β2AR-μDpTT	Beta-2 adrenergic receptor-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182327	pcDNA3-RIIα-μDpTT	PKA RIIα subunit-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182328	pcDNA3-H2B-μDpTT	Histone H2B-targeted Dronpa chromophore-removed FLINC (DrFLINC) pair. Fluctuating red-fluorescent label for live-cell super-resolution imaging.
182329	pcDNA3-Dronpa-Kras	Plasma membrane-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
182330	pcDNA3-Lifeact-Dronpa	Actin-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
182331	pcDNA3-RIIα-Dronpa	PKA RIIα subunit-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
182332	pcDNA3-AKAP79-Dronpa	AKAP79-targeted Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
182333	pGW-Dronpa-Cav1.2	L-type voltage-gated calcium channel α1C subunit tagged with Dronpa. Fluctuating green-fluorescent label for live-cell super-resolution imaging.
182334	pcDNA3-DrFLINC-AKAR-Kras	Genetically encoded fluorescent biosensor for super-resolution imaging of PKA activity; targeted to the plasma membrane. Compatible with multiplexed super-resolution imaging using a Dronpa-tagged POI.

Antibodies from Article