FNF-CAG-L-3xSV40pA-L-tdTomato shuttle vector
(Plasmid #182346)

• Purpose: PacI-flanked CAG-LSL expression cassette with a FNF for positive selection
• Depositing Lab: Mario Capecchi
• Publication: Mouse safe harbor locus targeting plasmids (unpublished)

Backbone

• Vector backbone: Custom
• Backbone manufacturer: Hyung-song Nam, Capecchi laboratory
• Vector type: Mammalian Expression, Mouse Targeting ; FLP / FRT
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tdTomato
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • ** 9 Pac1 3xpA loxP tdTomato ATG fixed.gb
• A portion of this plasmid was derived from a plasmid made by: FNF is from Addgene plasmid #22687. CAG promoter is from pCAGGS obtained from BCCM/LMBP. SV40 pA is from Clontech. tdTomato is from Roger Tsien. WPRE is from Miguel Ramalho-Santos. bGH pA is from Neal Copeland.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Release the insert with PacI.

Similar to the situation with some other plasmids in this collection, the missing four nucleotides at the 5' end of the WPRE renders it unrecognizable to SnapGene, but the expression vector is functional.