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Addgene

pCDNA_MET_D14_3Flag
(Plasmid #182495)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182495 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    pcDNA3.1+
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    MET
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    4029
  • Mutation
    Exon 14 deleted
  • Entrez Gene
    MET (a.k.a. AUTS9, DA11, DFNB97, HGFR, RCCP2, c-Met)
  • Tag / Fusion Protein
    • C-terminal 3xFlag tag (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA_MET_D14_3Flag was a gift from Paolo Comoglio (Addgene plasmid # 182495 ; http://n2t.net/addgene:182495 ; RRID:Addgene_182495)
  • For your References section:

    MET14 promotes a ligand-dependent, AKT-driven invasive growth. Cerqua M, Botti O, Arigoni M, Gioelli N, Serini G, Calogero R, Boccaccio C, Comoglio PM, Altintas DM. Life Sci Alliance. 2022 May 30;5(10). pii: 5/10/e202201409. doi: 10.26508/lsa.202201409. Print 2022 Oct. 10.26508/lsa.202201409 PubMed 35636967