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pcdna 3.1 DSG-3 Tailless Control
(Plasmid #182718)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182718 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 182718-D50 50 μg of DNA in Tris buffer $495
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DNA 182718-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pcdna 3.1
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    desmoglein-3
  • Alt name
    DSG3
  • Species
    M. musculus (mouse)
  • Mutation
    TSmod inserted at G726, downstream C-terminal tail of DSG-3 removed
  • GenBank ID
    NM_030596.4
  • Entrez Gene
    Dsg3 (a.k.a. bal)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This construct was created from DSG3 Flag (received from Andrew Kowalczyk)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.05.03.592394 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcdna 3.1 DSG-3 Tailless Control was a gift from Daniel Conway (Addgene plasmid # 182718 ; http://n2t.net/addgene:182718 ; RRID:Addgene_182718)

  • For your References section:

    Desmosomal cadherin tension loss in pemphigus vulgaris mediated by the inhibition of active RhoA at cell-cell adhesions. Jin X, Rosenbohm J, Kim E, Safa BT, Moghaddam AO, Seiffert-Sinha K, Leiker M, Jones E, Zhai H, Baddam SR, Minnick G, Huo Y, Wahl JK 3rd, Meng F, Huang C, Lim JY, Conway DE, Sinha AA, Yang R. iScience. 2025 Jul 9;28(8):113081. doi: 10.1016/j.isci.2025.113081. eCollection 2025 Aug 15. 10.1016/j.isci.2025.113081 PubMed 40740498
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