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pKC-4.06NMD-CR
(Plasmid #182968)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 182968 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    p3xFLAG-CMV-10
  • Backbone manufacturer
    Sigma
  • Backbone size w/o insert (bp) 6204
  • Total vector size (bp) 9931
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    3xFlag-FLuc-P2A-3xFlag-NLuc - beta-globin 39PTC
  • Alt name
    coincidence reporter NMD sensor
  • Alt name
    NMD coincidence reporter
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2307
  • GenBank ID
    M14574
  • Entrez Gene
    HBB (a.k.a. CD113t-C, ECYT6, beta-globin)
  • Promoter CMV
  • Tag / Fusion Protein
    • 3xFlag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoR1 (not destroyed)
  • 3′ cloning site EcoR1 (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer hgH-PA-R
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Prof. Lynne Maquat provided the beta-globin plasmid used to create pKC-4.06 from we created pKC-4.06NMD-CR

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKC-4.06NMD-CR was a gift from James Inglese (Addgene plasmid # 182968 ; http://n2t.net/addgene:182968 ; RRID:Addgene_182968)

  • For your References section:

    A coincidence reporter-gene system for high-throughput screening. Cheng KC, Inglese J. Nat Methods. 2012 Oct;9(10):937. doi: 10.1038/nmeth.2170. 10.1038/nmeth.2170 PubMed 23018994
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