pCJH019_SC101_pLAC_Cas12c(4)_Kan
(Plasmid
#183071)
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PurposeWild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183071 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSC101 ori
- Total vector size (bp) 8533
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameCas12c_4
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Alt nameCas12c
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SpeciesSynthetic
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Insert Size (bp)3657
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MutationWild-type
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCJH019_SC101_pLAC_Cas12c(4)_Kan was a gift from Jennifer Doudna (Addgene plasmid # 183071 ; http://n2t.net/addgene:183071 ; RRID:Addgene_183071) -
For your References section:
A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression. Huang CJ, Adler BA, Doudna JA. Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. 10.1016/j.molcel.2022.04.020 PubMed 35659325
