A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression.
Huang CJ, Adler BA, Doudna JA
Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. PubMed Article
Huang CJ, Adler BA, Doudna JA
Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 183069 | pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp | Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020. |
| 183070 | pCJH009_proD_Cas12c-sgRNA-GFP_CAM | Plasmid expressing Cas12c sgRNA targeting GFP (1) for in vivo interference assay in bacteria. |
| 183071 | pCJH019_SC101_pLAC_Cas12c(4)_Kan | Wild-type Cas12c_4 in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay. |
| 183072 | pCJH021_SC101_pLAC_Cas12c2_Kan | Wild-type Cas12c2 (Yan et al., 2019) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay. |
| 183073 | pCJH026_proD_Cas12c-multiplexing_CAM | Plasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression |
| 183074 | pCJH027_SC101_pLAC_LbCas12a_Kan | Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay. |
| 183075 | pCJH031_proD_LbCas12a-gRNA-GFP_CAM | Plasmid expressing Cas12a guide RNA targeting GFP (1) for in vivo interference assay in bacteria. |
| 183076 | pCJH059_proD_Cas12c_DualRNA-GFP_CAM | Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately. |
| 183077 | pCJH065_proD_Cas12c_DualRNA-GFP+HDVrz_CAM | Plasmid expressing Cas12c tracrRNA and GFP-targeting crRNA separately, with the crRNA ending with a HDV ribozyme sequence. |
| 183221 | Cas12c-sgRNA_BbsI_CAM | Cas12c sgRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly |
| 183222 | LbCas12a-gRNA_BbsI_CAM | LbCas12a gRNA containing BbsI restriction recognition sites in spacer sequence for Golden Gate Assembly |