pCJH027_SC101_pLAC_LbCas12a_Kan
(Plasmid #183074)

• Purpose: Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.
• Depositing Lab: Jennifer Doudna
• Publication: Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020.

Backbone

• Vector backbone: pSC101 ori
• Total vector size (bp): 8563
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: LbCas12a
• Species: Lachnospiraceae bacterium
• Insert Size (bp): 3687
• Mutation: Wild-type

Resource Information

• Supplemental Documents:
  • pCJH027_SC101_pLAC_LbCas12a_Kan.gbk
• A portion of this plasmid was derived from a plasmid made by: CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity. Chen JS, Ma E, Harrington LB, Da Costa M, Tian X, Palefsky JM, Doudna JA. Science. 2018 Apr 27;360(6387):436-439. doi: 10.1126/science.aar6245. Epub 2018 Feb 15. 10.1126/science.aar6245 PubMed 29449511

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The sequence of LbCas12a was from Addgene plasmid # 113431 ; http://n2t.net/addgene:113431 ; RRID:Addgene_113431