pCJH059_proD_Cas12c_DualRNA-GFP_CAM
(Plasmid #183076)

• Purpose: Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.
• Depositing Lab: Jennifer Doudna
• Publication: Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020.

Backbone

• Vector backbone: p15A ori
• Total vector size (bp): 3556
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol, 25 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas12c tracrRNA and pre-crRNA
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • pCJH059_proD_Cas12c_DualRNA-GFP_CAM.gbk
• A portion of this plasmid was derived from a plasmid made by: Functionally diverse type V CRISPR-Cas systems. Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA. Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6. 10.1126/science.aav7271 PubMed 30523077

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

A part of the Cas12c2 concatenated noncoding RNA sequence from Addgene plasmid # 120877 (http://n2t.net/addgene:120877 ; RRID:Addgene_120877) was used for tracrRNA expression.