pCJH026_proD_Cas12c-multiplexing_CAM
(Plasmid
#183073)
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PurposePlasmid expressing a Cas12c sgRNA targeting GFP and another sgRNA targeting RFP in one transcript for testing multiplexed repression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183073 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonep15A ori
- Total vector size (bp) 2443
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Vector typeBacterial Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameExpressing two Cas12c sgRNAs (targeting GFP or RFP) in one transcript
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SpeciesSynthetic
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCJH026_proD_Cas12c-multiplexing_CAM was a gift from Jennifer Doudna (Addgene plasmid # 183073 ; http://n2t.net/addgene:183073 ; RRID:Addgene_183073) -
For your References section:
A naturally DNase-free CRISPR-Cas12c enzyme silences gene expression. Huang CJ, Adler BA, Doudna JA. Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020. 10.1016/j.molcel.2022.04.020 PubMed 35659325
