pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp
(Plasmid #183069)

• Purpose: Bacterial protein expression plasmid of wild-type Cas12c_4. This is a R965H version of the Cas12c in Harrington et al., 2020.
• Depositing Lab: Jennifer Doudna
• Publication: Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4. doi: 10.1016/j.molcel.2022.04.020.

Backbone

• Vector backbone: Modified pET His10 MBP Asn10 TEV LIC cloning vector (2CT-10)
• Total vector size (bp): 9629
• Vector type: Bacterial Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: Cas12c_4
• Alt name: Cas12c
• Species: Synthetic
• Insert Size (bp): 3657
• Mutation: Wild-type
• Tags / Fusion Proteins:
  • His10 (N terminal on backbone)
  • MBP (N terminal on backbone)

Resource Information

• Supplemental Documents:
  • pCJH002_10xHis-MBP-TEVcs-Cas12c(4)_Amp.gbk
• A portion of this plasmid was derived from a plasmid made by: Harrington et al., 2020, Molecular Cell 79, 416–424 https://doi.org/10.1016/j.molcel.2020.06.022

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This Cas12c bacterial expression plasmid was originally created by Harrington et al., 2020 and now modified (R965H) to match with the wild-type protein sequence of Cas12c_4.