pLentiGuide_GFP_sgEPAS1#2
(Plasmid #183120)

• Purpose: pLentiGuide modified to express GFP and an sgRNA targeting EPAS1
• Depositing Lab: William Kaelin
• Publication: Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120403119. doi: 10.1073/pnas.2120403119. Epub 2022 Mar 31.

Backbone

• Vector backbone: pLentiGuide
• Backbone manufacturer: Addgene
• Backbone size w/o insert (bp): 9101
• Total vector size (bp): 9083
• Modifications to backbone: Modified to Express GFP
• Vector type: Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: EPAS1-2
• Alt name: Endothelial PAS domain-containing protein 1
• Alt name: HIF2-a
• Insert Size (bp): 18
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BSMBI (unknown if destroyed)
• 3′ cloning site: BSMBI (unknown if destroyed)
• 5′ sequencing primer: U6

Resource Information

• Supplemental Documents:
  • pLentiGuide sgEPAS1-2 Puro GFP.ape

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry