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Sensitivity of VHL mutant kidney cancers to HIF2 inhibitors does not require an intact p53 pathway.
Stransky LA, Vigeant SM, Huang B, West D, Denize T, Walton E, Signoretti S, Kaelin WG Jr
Proc Natl Acad Sci U S A. 2022 Apr 5;119(14):e2120403119. doi: 10.1073/pnas.2120403119. Epub 2022 Mar 31. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183112pLenti_EF1a_Flag_TP53_WTExpresses flag-tagged p53 in mammalian cells
183113pLenti_EF1a_Flag_TP53_P278AExpresses flag-tagged p53 with P278A mutation in mammalian cells
183114pLenti_EF1a_Flag_TP53_R248WExpresses flag-tagged p53 with R248W mutation in mammalian cells
183115pLenti_EF1a_Flag_TP53_R248W+P278AExpresses flag-tagged p53 with both R248W and P278A mutations in mammalian cells
183116pLenti_EF1a_EVEmpty vector control for pLenti_EF1a constructs
183117pLenti_EF1a_Flag_EGFPExpresses flag-tagged EGFP in mammalian cells
183118pLentiGuide_GFP_NT135pLentiGuide modified to express GFP and a non-targeting sgRNA
183119pLentiGuide_GFP_sgEPAS1#1pLentiGuide modified to express GFP and an sgRNa targeting EPAS1
183120pLentiGuide_GFP_sgEPAS1#2pLentiGuide modified to express GFP and an sgRNA targeting EPAS1
183121pLentiGuide_mCherry_NT135pLentiGuide modified to express mCherry and a non-targeting sgRNA
183122pLentiGuide_mCherry_sgEPAS1#1pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1
183123pLentiGuide_mCherry_sgEPAS1#2pLentiGuide modified to express mCherry and an sgRNA targeting EPAS1
183180pLentiCRISPR_zeo_sgTP53pLentiCRISPR that is selectable with zeocin with an sgRNA targeting TP53
183181pLentiCRISPR_zeo_sgNT135pLentiCRISPR that is selectable with zeocin with a non-targeting sgRNA
187818pLentiCRISPR_Puro_sgAAVS1pLentiCRISPR that is selectable with puromycin with an sgRNA targeting AAVS1
187819pLentiCRISPR_Puro_sgTP53pLentiCRISPR that is selectable with puromycin with an sgRNA targeting TP53

Antibodies from Article