NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1
(Plasmid
#183399)
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PurposeDual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 183399 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepCDNA3
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameGI-VP16-IRES-GAL4DBD-NanoLuc-FKF1
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SpeciesSynthetic
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1 was a gift from Alice Ting (Addgene plasmid # 183399 ; http://n2t.net/addgene:183399 ; RRID:Addgene_183399) -
For your References section:
A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells. Erdenee E, Ting AY. ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 10.1021/acssynbio.1c00597. Epub 2022 Mar 7. 10.1021/acssynbio.1c00597 PubMed 35254056
