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A Dual-Purpose Real-Time Indicator and Transcriptional Integrator for Calcium Detection in Living Cells.

Erdenee E, Ting AY
ACS Synth Biol. 2022 Mar 18;11(3):1086-1095. doi: 10.1021/acssynbio.1c00597. Epub 2022 Mar 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
183399NLS-GI-VP16-IRES-NLS-GAL4DBD-NanoLuc-FKF1Dual expression of GI-VP16 and GAL4DBD-NanoLuc-FKF1 in the nucleus
183400NLS-GI-VP64-IRES-NLS-GAL4DBD-M13-SmBiT-FKF1Dual expression of GI-VP64 and GAL4DBD-M13-SmBiT-FKF1 in the nucleus
183401NLS-LgBiT-CaMExpresses nuclear-localized LgBiT-CaM
183402NLS-GI-VP16-IRES-NLS-GAL4DBD-FKBP-SMBiT-FKF1Dual expression of GI-VP16 and GAL4DBD-FKBP-SmBiT-FKF1 in the nucleus
183403NLS-LgBiT-FRBExpresses nuclear-localized LgBiT-FRB
183404NLS-GI-VP16-IRES-NLS-GAL4DBD-GID-SmBiT-FKF1Dual expression of GI-VP16 and GAL4DBD-GID-SmBiT-FKF1 in the nucleus
183405NLS-LgBiT-GAI Expresses nuclear-localized LgBiT-GAI

Antibodies from Article