pX459-HypaCas9-MYH10_sgRNA
(Plasmid #183887)

• Purpose: pX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.
• Depositing Lab: Alisa Piekny
• Publication: Husser et al Open Biol. 2022 Nov;12(11):220247. doi: 10.1098/rsob.220247. Epub 2022 Nov 23.

Backbone

• Vector backbone: pX459V2.0-HypaCas9
• Total vector size (bp): 9178
• Vector type: Mammalian Expression, CRISPR
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MYH10 sgRNA spacer
• Alt name: Myosin heavy chain 10
• gRNA/shRNA sequence: GTTCTCTGCGCCATTGTAAA
• Species: H. sapiens (human)
• Entrez Gene: MYH10 (a.k.a. NMMHC-IIB, NMMHCB)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BbsI (destroyed during cloning)
• 3′ cloning site: BbsI (destroyed during cloning)
• 5′ sequencing primer: gagggcctatttcccatgattcc
• 3′ sequencing primer: ggccatttaccgtaagttatgtaacg

Resource Information

• Supplemental Documents:
  • pX459V2.0-HypaCas9-sgRNA_MYH10.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry