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Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA.
Husser MC, Ozugergin I, Resta T, Martin VJJ, Piekny AJ
Open Biol. 2022 Nov;12(11):220247. doi: 10.1098/rsob.220247. Epub 2022 Nov 23. PubMed Article

Plasmids from Article

ID Plasmid Purpose
183834pODN-mNG-ANLNRepair template for the N-terminal tagging of anillin with mNeonGreen in human cells using CRISPR/Cas9.
183835pODN-mNG-ECT2Repair template for the N-terminal tagging of Ect2 with mNeonGreen in human cells using CRISPR/Cas9.
183836pODN-mNG-RHOARepair template for the N-terminal tagging of RhoA with mNeonGreen in human cells using CRISPR/Cas9.
183837pODN-mNG-CfANLNRepair template for the N-terminal tagging of anillin with mNeonGreen in canine cells using CRISPR/Cas9.
183866pODN-H2BC11-mR2Repair template for the C-terminal tagging of H2B histones with mRuby2 in human cells using CRISPR/Cas9.
183867pODN-H2BC11-TagBFPRepair template for the C-terminal tagging of H2B histones with mTagBFP in human cells using CRISPR/Cas9.
183868pODN-mR2-ACTBRepair template for the N-terminal tagging of beta-actin with mRuby2 in human cells using CRISPR/Cas9.
183869pODN-mR2-MYH10Repair template for the N-terminal tagging of myosin heavy chain 10 with mRuby2 in human cells using CRISPR/Cas9.
183870pODN-AAVS1-mNG-CAAXRepair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
183871pODN-AAVS1-mR2-CAAXRepair template for the stable expression of a mRuby2-CAAX fusion in human cells using CRISPR/Cas9.
183872pX459V2.0-HypaCas9-mRuby2pX459V2.0 plasmid expressing HypaCas9 with P2A-mRuby2 and T2A-Puro, with a sgRNA cloning site for CRISPR editing in mammalian cells.
183873pX459-HypaCas9-ECT2_sgRNApX459V2.0-HypaCas9 plasmid with ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.
183874pX459-HypaCas9-ANLN_sgRNApX459V2.0-HypaCas9 plasmid with ANLN sgRNA for N-terminal tagging of anillin in human cells.
183875pX459-HypaCas9-mR2-ECT2_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ECT2 sgRNA for N-terminal tagging of Ect2 in human cells.
183876pX459-HypaCas9-mR2-ANLN_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ANLN sgRNA for N-terminal tagging of anillin in human cells.
183877pX459-HypaCas9-mR2-RHOA_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and RHOA sgRNA for N-terminal tagging of RhoA in human cells.
183878pX459-HypaCas9-RHOA_sgRNApX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.
183879pX459-HypaCas9-mR2-CfANLN_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.
183880pX459-HypaCas9-CfANLN_sgRNApX459V2.0-HypaCas9 plasmid with cfANLN sgRNA for N-terminal tagging of anillin in canine (Canis familiaris) cells.
183881pX459-HypaCas9-mR2-H2BC11_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.
183883pX459-HypaCas9-H2BC11_sgRNApX459V2.0-HypaCas9 plasmid with H2BC11 sgRNA for C-terminal tagging of H2B histones in human cells.
183884pX459-HypaCas9-mR2-ACTB_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and ACTB sgRNA for N-terminal tagging of beta-actin in human cells.
183885pX459-HypaCas9-ACTB_sgRNApX459V2.0-HypaCas9 plasmid with ACTB sgRNA for N-terminal tagging of beta-actin in human cells.
183886pX459-HypaCas9-mR2-MYH10_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.
183887pX459-HypaCas9-MYH10_sgRNApX459V2.0-HypaCas9 plasmid with MYH10 sgRNA for N-terminal tagging of myosin heavy chain 10 in human cells.
183888pX459-HypaCas9-mR2-TUBA1B_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.
183889pX459-HypaCas9-TUBA1B_sgRNApX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.
183890pX459-HypaCas9-mR2-AAVS1_sgRNApX459V2.0-HypaCas9 plasmid with mRuby2 reporter and sgRNA targeting the AAVS1 in human cells.
183891pX459-HypaCas9-AAVS1_sgRNApX459V2.0-HypaCas9 plasmid with sgRNA targeting the AAVS1 in human cells.

Antibodies from Article