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(Plasmid #183872)


Item Catalog # Description Quantity Price (USD)
Plasmid 183872 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size (bp) 9175
  • Modifications to backbone
    Insertion of P2A-mRuby2 for expression with HypaCas9 and T2A-Puro
  • Vector type
    Mammalian Expression, CRISPR
  • Promoter Cbh
  • Selectable markers
  • Tag / Fusion Protein
    • HypaCas9-P2A-mRuby2-T2A-Puro

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pX459V2.0-HypaCas9-mRuby2 was a gift from Alisa Piekny (Addgene plasmid # 183872 ; ; RRID:Addgene_183872)
  • For your References section:

    Cytokinetic diversity in mammalian cells is revealed by the characterization of endogenous anillin, Ect2 and RhoA. Husser MC, Ozugergin I, Resta T, Martin VJJ, Piekny AJ. Open Biol. 2022 Nov;12(11):220247. doi: 10.1098/rsob.220247. Epub 2022 Nov 23. 10.1098/rsob.220247 PubMed 36416720