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(Plasmid #184060)


Item Catalog # Description Quantity Price (USD)
Plasmid 184060 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pSmart-LowCopy/pT2KXIGdin hybrid
  • Backbone manufacturer
    Lucigen Corp
  • Backbone size w/o insert (bp) 5767
  • Vector type
    Zebrafish transient transgenesis

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    Phage P1 (CRE), H. sapiens (ERT2)
  • Insert Size (bp)
  • Mutation
    ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Zf-Hsp70-4

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site StuI (destroyed during cloning)
  • 3′ cloning site XhoI (destroyed during cloning)
  • 5′ sequencing primer T7
  • 3′ sequencing primer SVErev2
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    ERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

CRE: YP_006472; ERT2: NP_000116;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT2h70CRET(#325) was a gift from Sophie Vriz (Addgene plasmid # 184060 ; ; RRID:Addgene_184060)
  • For your References section:

    Optical Control of Tumor Induction in the Zebrafish. Feng Z, Nam S, Hamouri F, Aujard I, Ducos B, Vriz S, Volovitch M, Jullien L, Lin S, Weiss S, Bensimon D. Sci Rep. 2017 Aug 23;7(1):9195. doi: 10.1038/s41598-017-09697-x. 10.1038/s41598-017-09697-x PubMed 28835665