pCSGFPnERT(#205)
(Plasmid
#184065)
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Purposecyclofen-inducible GFP nuclear relocation via mammalian cell transfection or mRNA synthesis
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Depositing Lab
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Depositing OrganizationCollege de France
Located in France -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184065 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 184065-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 184065-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepCS2
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Backbone manufacturerDL Turner (Turner & Weintraub, 1994, G&Dev 8:1434)
- Backbone size w/o insert (bp) 4063
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameeGFP-nls-ERT2
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SpeciesAequora victoria (GFP), SV40 (nls), H. sapiens (ERT2)
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Insert Size (bp)1753
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MutationERT2: G400V, M543A, L544A, deleted 1-281;
- Promoter sCMV+SP6
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site ClaI (not destroyed)
- 3′ cloning site BsaBI (not destroyed)
- 5′ sequencing primer SP6
- 3′ sequencing primer SVLrev34
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byERT2 (Feil and al 1997 BBRC 237:752) received from Daniel Metzger & Pierre Chambon in may 2004
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
eGPF: AAB02572; nls; ERT2: NP_000116;
DNA (Catalog # 184065-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 184065-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCSGFPnERT(#205) was a gift from Sophie Vriz (Addgene plasmid # 184065 ; http://n2t.net/addgene:184065 ; RRID:Addgene_184065) -
For your References section:
Photocontrol of protein activity in cultured cells and zebrafish with one- and two-photon illumination. Sinha DK, Neveu P, Gagey N, Aujard I, Benbrahim-Bouzidi C, Le Saux T, Rampon C, Gauron C, Goetz B, Dubruille S, Baaden M, Volovitch M, Bensimon D, Vriz S, Jullien L. Chembiochem. 2010 Mar 22;11(5):653-63. doi: 10.1002/cbic.201000008. 10.1002/cbic.201000008 PubMed 20187057
