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(Plasmid #184089)


Item Catalog # Description Quantity Price (USD)
Plasmid 184089 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pT22i (pT2AL200R150G derivative)
  • Backbone manufacturer
    modified by M Volovitch from pT2AL200R150 of A Urasaki; Urasaki et al 2006, Genetics 174:639)
  • Backbone size w/o insert (bp) 8031
  • Vector type
    Zebrafish transgenesis (blue eyes)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (myc), G. gallus (chkEn2), H. sapiens (ERT2), PTV-1 (P2A), Discosoma sp. (mCherry)
  • Insert Size (bp)
  • Mutation
    chkEn2: W247S, F248R; ERT2: G400V, M543A, L544A, deleted 1-281;
  • Promoter Zf-Ubi
  • Tag / Fusion Protein
    • 5xMyc (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site AscI (not destroyed)
  • 5′ sequencing primer ubidir
  • 3′ sequencing primer SVLrev34
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthetic ERT2

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5myc; chkEn2: NP_001254648; ERT2: NP_000116; P2A: AIE57569; mCherry: AY678264 (polycistronic mRNA: mCherry cytoplasmic marker of expression) + gCRY:CFP as a marker of transgenesis;

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pT22Ubm5En2SRE4PCh(#302) was a gift from Sophie Vriz (Addgene plasmid # 184089 ; ; RRID:Addgene_184089)
  • For your References section:

    Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain. Rampon C, Gauron C, Lin T, Meda F, Dupont E, Cosson A, Ipendey E, Frerot A, Aujard I, Le Saux T, Bensimon D, Jullien L, Volovitch M, Vriz S, Joliot A. Development. 2015 May 15;142(10):1840-9. doi: 10.1242/dev.114181. Epub 2015 Apr 29. 10.1242/dev.114181 PubMed 25926358