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Anti-Ankyrin-B [N105/13R]
(Antibody #184191)

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Western Blot image by James Trimmer, UC Davis

Image 1 (of 4): Western Blot
Crude rat membranes immunoblotted against Anti-Ankyrin-B hybridoma-derived antibody from … View details
Credit: James Trimmer, UC Davis

Immunohistochemistry image by Zhuhao Wu, Weill Cornell Medicine

Image 2 (of 4): Immunohistochemistry
Delipidated mouse brain tissue was stained with Anti-Ankyrin-B [N105/13R]. View details
Credit: Zhuhao Wu, Weill Cornell Medicine

Immunohistochemistry image by James Trimmer, UC Davis

Image 3 (of 4): Immunohistochemistry
Free-floating rat brain sections were fixed and stained with purified recombinant Anti-An… View details
Credit: James Trimmer, UC Davis

Western Blot image by James Trimmer, UC Davis

Image 4 (of 4): Western Blot
Crude rat membranes immunoblotted against Anti-Ankyrin-B purified recombinant antibody pr… View details
Credit: James Trimmer, UC Davis

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Item Catalog # Description Quantity Price (USD)
Recombinant Antibody 184191-rAb 100 µg of purified recombinant antibody $250 *
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Recombinant Antibody trial size 184191-rAb.T 20 µg of purified recombinant antibody $85 *
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Features

Production & Usage

  • Production Plasmid
    Anti-Ankyrin-B [N105/13R]
  • Purification
    Purified from cell culture supernatant using Protein A or Protein G affinity columns followed by a buffer exchange with centrifugal columns.
  • Storage Buffer
    PBS + 1 mM sodium azide

Delivery

  • Concentration
    0.9–1.1 mg/mL
  • Storage
    Upon receipt, prepare single use aliquots and store at -20 °C until use. Avoid multiple freeze thaw cycles.
  • Shipment
    Blue ice or room temp

Resource Information

  • Persistent Resource Identifier
    RRID:AB_2909555

Terms and Licenses

Target Antigen

Protein Ankyrin-2
Antigen Description Amino acids 203–496 (ankyrin repeats 6 to 14) of human Ankyrin-B
Antigen Amino Acid Sequence
DDTKSAALLLQNDHNADVQSKMMVNRTTESGFTPLHIAAHYGNVNVATLLLNRGAAVDFTARNGITPLHVASKRGNTNMVKLLLDRGGQIDAKTRDGLTPLHCAARSGHDQVVELLLERGAPLLARTKNGLSPLHMAAQGDHVECVKHLLQHKAPVDDVTLDYLTALHVAAHCGHYRVTKLLLDKRANPNARALNGFTPLHIACKKNRIKVMELLVKYGASIQAITESGLTPIHVAAFMGHLNIVLLLLQNGASPDVTNIRGETALHMAARAGQVEVVRCLLRNGALVDARARE
Species H. sapiens (human)
Additional Species Reactivity M. musculus (mouse),  R. norvegicus (rat)
Gene ANK2
Alternative Names
  • Ankyrin-B
  • Brain ankyrin
  • Non-erythroid ankyrin
External References

Applications (3)

Information
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Western Blot

Recommended
1 image
Western Blot image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
1 image
Western Blot image by James Trimmer, UC Davis
Submitted By: James Trimmer, UC Davis
Addgene Partner
Antibody Type
Recombinant
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
1 image
Western Blot image by UC Davis/NIH NeuroMab Facility
Submitted By: UC Davis/NIH NeuroMab Facility
Antibody Type
Hybridoma
Sample Species
R. norvegicus (rat)
Result
Pass
View Details
How to cite this antibody ( Back to top)

These antibodies were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the antibodies were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Anti-Ankyrin-B [N105/13R] - from James Trimmer (Addgene antibody # 184191 ; http://n2t.net/addgene:184191 ; RRID:AB_2909555)

  • For your References section:

    A toolbox of IgG subclass-switched recombinant monoclonal antibodies for enhanced multiplex immunolabeling of brain. Andrews NP, Boeckman JX, Manning CF, Nguyen JT, Bechtold H, Dumitras C, Gong B, Nguyen K, van der List D, Murray KD, Engebrecht J, Trimmer JS. Elife. 2019 Jan 22;8. pii: 43322. doi: 10.7554/eLife.43322. 10.7554/eLife.43322 PubMed 30667360