pCMV10-3xFLAG-prs-XK
(Plasmid #184369)

• Purpose: Express human XK in Expi293 cells
• Depositing Lab: Karin Reinisch
• Publication: Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e202202030. doi: 10.1083/jcb.202202030. Epub 2022 Mar 31.

Backbone

• Vector backbone: pCMV10
• Backbone size w/o insert (bp): 6281
• Total vector size (bp): 7616
• Modifications to backbone: digested with NotI and BamHI
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human XK
• Alt name: XK-related protein1
• Species: H. sapiens (human)
• Insert Size (bp): 1335
• GenBank ID: NC_000023.11
• Entrez Gene: XK (a.k.a. KX, NA, NAC, X1k, XKR1)
• Promoter: CMV
• Tag / Fusion Protein:
  • 3xFLAG-PreScission cleavage site (N terminal on backbone)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ccaggggccccttgcggccgcgATGAAATTCCCGGCCTCG
• 3′ sequencing primer: agggatgccacccgggatccTTAAGCAGAGCAGAGATCTTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry