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pAT15547_PEmax-SuperFi-Cas9
(Plasmid #184377)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 184377 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 184377-D50 50 μg of DNA in Tris buffer $495
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DNA 184377-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    pUC19
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    PEmax-SuperFi-Cas9
  • Alt name
    SuperFi-Cas9(H840A) fused with the codon optimized MMLV reverse transcriptase - prime editor PEmax
  • Species
    S. pyogenes
  • Mutation
    H840A, R221K, N394K, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, K1031D
  • Promoter CMV
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • c-myc NLS (C terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAT15547_PEmax-SuperFi-Cas9 was a gift from Ervin Welker (Addgene plasmid # 184377 ; http://n2t.net/addgene:184377 ; RRID:Addgene_184377)

  • For your References section:

    SuperFi-Cas9 exhibits remarkable fidelity but severely reduced activity yet works effectively with ABE8e. Kulcsar PI, Talas A, Ligeti Z, Krausz SL, Welker E. Nat Commun. 2022 Nov 11;13(1):6858. doi: 10.1038/s41467-022-34527-8. 10.1038/s41467-022-34527-8 PubMed 36369279
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