pCAG-H2B-GFP
(Plasmid #184777)

• Purpose: Expresses H2B-GFP in human pluripotent stem cells
• Depositing Lab: Ivana Barbaric
• Publication: Price et al Curr Protoc. 2022 May;2(5):e435. doi: 10.1002/cpz1.435.

Backbone

• Vector backbone: pCAG
• Total vector size (bp): 7625
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: H2BC11
• Alt name: Human histone H2B
• Alt name: H2BJ; H2B/r; H2BFR; HIST1H2BJ
• Species: H. sapiens (human)
• Insert Size (bp): 380
• GenBank ID: X00088
• Entrez Gene: H2BC11 (a.k.a. H2B/r, H2BFR, H2BJ, HIST1H2BJ)
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (unknown if destroyed)
• 3′ cloning site: BamHI (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: EGFP-N

Resource Information

• Supplemental Documents:
  • pCAG-MCS-H2B-GFP-puro ape.seq
• A portion of this plasmid was derived from a plasmid made by: Addgene plasmid #11680

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

H2B-EGFP sequence from plasmid #11680 was excised at NheI and NotI sites and ligated into pCAG vector with compatible overhangs.