pMRX-IP-GFP-LC3-mRuby3
(Plasmid
#184898)
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PurposeStable expression of GFP-LC3-mRuby3 in mammalian cells to measure autophagic flux
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Depositing Lab
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Depositing OrganizationThe University of Tokyo
Located in Japan -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 184898 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 184898-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 184898-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepMRX-IP
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameLC3B
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SpeciesR. norvegicus (rat)
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Insert Size (bp)357
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Tags
/ Fusion Proteins
- EGFP (N terminal on insert)
- mRuby3 (C terminal on insert)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 184898-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 184898-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMRX-IP-GFP-LC3-mRuby3 was a gift from Noboru Mizushima (Addgene plasmid # 184898 ; http://n2t.net/addgene:184898 ; RRID:Addgene_184898) -
For your References section:
Evolution and insights into the structure and function of the DedA superfamily containing TMEM41B and VMP1. Okawa F, Hama Y, Zhang S, Morishita H, Yamamoto H, Levine TP, Mizushima N. J Cell Sci. 2021 Apr 15;134(8). pii: 237813. doi: 10.1242/jcs.255877. Epub 2021 Apr 28. 10.1242/jcs.255877 PubMed 33771928
