|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||1851||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 4700
Vector typeBacterial Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Copy numberHigh Copy
Alt nameFas death domain-associated protein
SpeciesM. musculus (mouse)
Insert Size (bp)2500
/ Fusion Protein
- myc (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (destroyed during cloning)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer pCAG-F (Common Sequencing Primers)
Terms and Licenses
Amino acids 1-739 of DAXX (entire ORF) PCR'd with myc tag at 3' end, cut with EcoRV, and cloned into blunted XhoI sites of pCAGGS. To be used for eukaryotic expression. Construct has ATG and Kozak.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:DAXX-myc was a gift from Philip Leder (Addgene plasmid # 1851 ; http://n2t.net/addgene:1851 ; RRID:Addgene_1851)
For your References section:Homeodomain-interacting protein kinase 1 modulates Daxx localization, phosphorylation, and transcriptional activity. Ecsedy JA, Michaelson JS, Leder P. Mol Cell Biol 2003 Feb;23(3):950-60. 10.1128/MCB.23.3.950-960.2003 PubMed 12529400