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pCS2+ Zf GFP-vcla
(Plasmid #185410)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 185410 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone size w/o insert (bp) 4095
  • Total vector size (bp) 8035
  • Vector type
    Mammalian Expression ; In vitro synthesis of mRNA

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    vinculin a
  • Alt name
    vcla, vcl
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
  • Entrez Gene
    vcla (a.k.a. hm:zehn2160, im:6902790, vcl)
  • Tag / Fusion Protein
    • eGFP (N terminal on backbone)

Cloning Information

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr Johan de Rooij, University Medical Center Utrecht

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCS2+ Zf GFP-vcla was a gift from Nicolas David (Addgene plasmid # 185410 ; ; RRID:Addgene_185410)
  • For your References section:

    Guidance by followers ensures long-range coordination of cell migration through alpha-catenin mechanoperception. Boutillon A, Escot S, Elouin A, Jahn D, Gonzalez-Tirado S, Starruss J, Brusch L, David NB. Dev Cell. 2022 May 17. pii: S1534-5807(22)00329-X. doi: 10.1016/j.devcel.2022.05.001. 10.1016/j.devcel.2022.05.001 PubMed 35613615