KBB11
(Plasmid
#185422)
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PurposeFull-length KAP60/SRP1
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185422 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS314
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Vector typeYeast Expression
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Selectable markersTRP1
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKAP60
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SpeciesS. cerevisiae (budding yeast)
- Promoter endogenous
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please note: KAP60/SRP1 is in the opposite orientation of other plasmids in the deposit making it downstream of the T3 promoter as opposed to downstream of T7 promoter.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
KBB11 was a gift from Ken Belanger & Laura Davis (Addgene plasmid # 185422 ; http://n2t.net/addgene:185422 ; RRID:Addgene_185422) -
For your References section:
Genetic and physical interactions between Srp1p and nuclear pore complex proteins Nup1p and Nup2p. Belanger KD, Kenna MA, Wei S, Davis LI. J Cell Biol. 1994 Aug;126(3):619-30. doi: 10.1083/jcb.126.3.619. 10.1083/jcb.126.3.619 PubMed 8045927
