pBig1a zz TEV YBBR RAP1 ZZ TEV TRF2
(Plasmid #185449)

• Purpose: Coexpresses human RAP1 with an MBP affinity tag, YBBR site, and TEV site, and human TRF2 with a zz tag and TEV site
• Depositing Lab: Ahmet Yildiz
• Publication: Jack et al Dev Cell. 2022 Jan 24;57(2):277-290.e9. doi: 10.1016/j.devcel.2021.12.017.

Backbone

• Vector backbone: pBig1a
• Backbone size w/o insert (bp): 6500
• Total vector size (bp): 11580
• Vector type: Insect Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin and Streptomycin, 100 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: TRF2
• Species: H. sapiens (human)
• Insert Size (bp): 1626
• GenBank ID: NP_005643.2
• Entrez Gene: TERF2 (a.k.a. TRBF2, TRF2)
• Tag / Fusion Protein:
  • ZZ (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CACCGTCAGCCGGGGCTGAACTTTCGTTTTC
• 3′ sequencing primer: CCAGCAGAAGATGCTGCGCTTCCTGGAGAGC

Gene/Insert 2

• Gene/Insert name: RAP1
• Species: H. sapiens (human)
• Insert Size (bp): 1200
• GenBank ID: NM_018975.4
• Entrez Gene: TERF2IP (a.k.a. DRIP5, RAP1)
• Tags / Fusion Proteins:
  • MBP (N terminal on insert)
  • YBBR (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CTA CGT GAA GGA AAA TGC CC
• 3′ sequencing primer: CGA GTG CTG CGT GAG CGA GC

Resource Information

• Supplemental Documents:
  • pbig1a-mbp_tev_ybbr_rap1-zz_trf2.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry