CamKII-LGI1-pHmScarlet
(Plasmid
#185538)
-
PurposeExpresses LGI1-pHmScarlet under the CamKII promoter
-
Depositing Lab
-
Publication
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 185538 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
-
Vector backboneCamKII
- Total vector size (bp) 12026
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nameLGI1
-
Alt nameLeucine-rich glioma-inactivated protein 1
-
SpeciesR. norvegicus (rat)
-
Entrez GeneLgi1
- Promoter CamKII
-
Tag
/ Fusion Protein
- pHmScarlet (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer agtattgtgtatataaggc (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://www.biorxiv.org/content/10.1101/2022.07.03.498586v2 for bioRxiv preprint.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
CamKII-LGI1-pHmScarlet was a gift from Jaime de Juan-Sanz (Addgene plasmid # 185538 ; http://n2t.net/addgene:185538 ; RRID:Addgene_185538) -
For your References section:
Activity-driven synaptic translocation of LGI1 controls excitatory neurotransmission. Cuhadar U, Calzado-Reyes L, Pascual-Caro C, Hallerman S, Hoppa MB, de Juan-Sanz J. Cell Reports 43, 5; 114186, May 28, 2024 10.1016/j.celrep.2024.114186
