FUGW-UBC-EGFP-2A-dnVAMP2
(Plasmid #185723)

• Purpose: Lentiviral expression of GFP and dominant-negative VAMP2 from UbC promoter
• Depositing Lab: Jun Ding
• Publication: Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. doi: 10.1038/s41593-023-01345-0. Epub 2023 May 29.

Backbone

• Vector backbone: FUGW (Plasmid #14883)
• Backbone size w/o insert (bp): 9930
• Total vector size (bp): 291
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: VAMP-2
• Alt name: SYB2, VAMP2
• Species: H. sapiens (human)
• Insert Size (bp): 290
• Mutation: deleted AA 97-116
• GenBank ID: NM_014232.3
• Entrez Gene: VAMP2 (a.k.a. NEDHAHM, SYB2, VAMP-2)
• Promoter: hUbC

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • C.90-FUGW-UBC-EGFP-2A-dnSNARE.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry